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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
27/08/2020 |
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Actualizado : |
21/05/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
BARAIBAR, S.; GARCIA, R.; SILVA, P.; LADO, B.; CASTRO, A.; GUTIÉRREZ , L.; KAVANOVÁ, M.; QUINCKE, M.; BHAVANI , S.; RANDHAWA, M.S.; GERMAN, S. |
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Afiliación : |
SILVINA BARAIBAR PEDERSEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RICHARD ANSELMO GARCIA USUCA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA PAULA SILVA VILLELLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; BETINA LADO, Facultad de Agronomía, Universidad de la República, Garzón 780, 12900 Montevideo, Uruguay.; AIREL CASTRO, Facultad de Agronomía, Universidad de la República, Estación Experimental Dr. Mario A. Cassinoni, Ruta 3, km 363, 60000 Paysandú, Uruguay; LUCIA GUTIÉRREZ, Department of Agronomy, University of Wisconsin–Madison, 1575, Linden Dr, Madison, WI 53706, USA.; MONIKA KAVANOVÁ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN CONRADO QUINCKE WALDEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SRIDHAR BHAVANI, International Maize and Wheat Improvement Center (CIMMYT), Km. 45, Carretera, México-Veracruz, El Batán, CP 56237 Texcoco, Edo. de México, Mexico.; MANDEEP S. RANDHAWA, International Maize and Wheat Improvement Center (CIMMYT), World Agroforestry Centre (ICRAF), United Nations Avenue, Gigiri, P.O. Box 1041, Nairobi 00621, Kenya.; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
QTL of resistance to Ug99 and other stem rust pathogen races in bread wheat. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Molecular Breeding, 1 August 2020, Volume 40, Issue 8, Article number 82. DOI: https://doi.org/10.1007/s11032-020-01153-5 |
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DOI : |
10.1007/s11032-020-01153-5 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 26 June 2019/ Accepted 23 October 2019/ Published 15 August 2020. |
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Contenido : |
Abstract:
Most wheat cultivars planted worldwide are susceptible to the stem rust Ug99 race group. To prepare for the potential spread of these races into South America, we aimed to identify genomic regions responsible for resistance to Ug99 race group in germplasm adapted to South America. Two RIL populations from a cross between a stem rust susceptible parent ?Baguette 13? and resistant local parents ?INIA Tero? and ?BR23//CEP19/PF85490? were developed. Phenotypical evaluation was completed at the seedling stage in Uruguay and under field conditions in Uruguay and Kenya. Both RIL populations were genotyped using the GBS approach. Besides Sr24, three other resistance loci in ?INIA Tero? were detected on chromosomes 2B, 6A, and 7B. All four QTL were effective to local races, whereas only the QTL on chromosome 2B was effective against the Ug99 race group. Besides Sr31, ?BR23//CEP19/PF85490? also carries two other stem rust resistance loci on chromosomes 2B and 6A. All three explained the resistance in Uruguay, while only the QTL on 2B was effective to Ug99 in Kenya. The physical location suggested that the QTL identified on chromosome 2B in both populations may correspond to Sr28, which was confirmed using specific molecular markers. Further studies are needed to determine the relationship between QTL for resistance to local races identified on chromosomes 6A and 7B and previously reported resistance genes and QTL. The results of this study are highly relevant for breeding wheat cultivars with diverse and durable resistance to stem rust. MenosAbstract:
Most wheat cultivars planted worldwide are susceptible to the stem rust Ug99 race group. To prepare for the potential spread of these races into South America, we aimed to identify genomic regions responsible for resistance to Ug99 race group in germplasm adapted to South America. Two RIL populations from a cross between a stem rust susceptible parent ?Baguette 13? and resistant local parents ?INIA Tero? and ?BR23//CEP19/PF85490? were developed. Phenotypical evaluation was completed at the seedling stage in Uruguay and under field conditions in Uruguay and Kenya. Both RIL populations were genotyped using the GBS approach. Besides Sr24, three other resistance loci in ?INIA Tero? were detected on chromosomes 2B, 6A, and 7B. All four QTL were effective to local races, whereas only the QTL on chromosome 2B was effective against the Ug99 race group. Besides Sr31, ?BR23//CEP19/PF85490? also carries two other stem rust resistance loci on chromosomes 2B and 6A. All three explained the resistance in Uruguay, while only the QTL on 2B was effective to Ug99 in Kenya. The physical location suggested that the QTL identified on chromosome 2B in both populations may correspond to Sr28, which was confirmed using specific molecular markers. Further studies are needed to determine the relationship between QTL for resistance to local races identified on chromosomes 6A and 7B and previously reported resistance genes and QTL. The results of this study are highly relevant for breeding wh... Presentar Todo |
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Palabras claves : |
GENETIC RESISTANCE; PUCCINIA GRAMINIS F. SP. TRITICI; QTL MAPPING; TRITICUM AESTIVUM. |
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Thesagro : |
RESISTENCIA GENETICA; TRIGO. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02649naa a2200337 a 4500
001 1061286
005 2021-05-21
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s11032-020-01153-5$2DOI
100 1 $aBARAIBAR, S.
245 $aQTL of resistance to Ug99 and other stem rust pathogen races in bread wheat.$h[electronic resource]
260 $c2020
500 $aArticle history: Received 26 June 2019/ Accepted 23 October 2019/ Published 15 August 2020.
520 $aAbstract: Most wheat cultivars planted worldwide are susceptible to the stem rust Ug99 race group. To prepare for the potential spread of these races into South America, we aimed to identify genomic regions responsible for resistance to Ug99 race group in germplasm adapted to South America. Two RIL populations from a cross between a stem rust susceptible parent ?Baguette 13? and resistant local parents ?INIA Tero? and ?BR23//CEP19/PF85490? were developed. Phenotypical evaluation was completed at the seedling stage in Uruguay and under field conditions in Uruguay and Kenya. Both RIL populations were genotyped using the GBS approach. Besides Sr24, three other resistance loci in ?INIA Tero? were detected on chromosomes 2B, 6A, and 7B. All four QTL were effective to local races, whereas only the QTL on chromosome 2B was effective against the Ug99 race group. Besides Sr31, ?BR23//CEP19/PF85490? also carries two other stem rust resistance loci on chromosomes 2B and 6A. All three explained the resistance in Uruguay, while only the QTL on 2B was effective to Ug99 in Kenya. The physical location suggested that the QTL identified on chromosome 2B in both populations may correspond to Sr28, which was confirmed using specific molecular markers. Further studies are needed to determine the relationship between QTL for resistance to local races identified on chromosomes 6A and 7B and previously reported resistance genes and QTL. The results of this study are highly relevant for breeding wheat cultivars with diverse and durable resistance to stem rust.
650 $aRESISTENCIA GENETICA
650 $aTRIGO
653 $aGENETIC RESISTANCE
653 $aPUCCINIA GRAMINIS F. SP. TRITICI
653 $aQTL MAPPING
653 $aTRITICUM AESTIVUM
700 1 $aGARCIA, R.
700 1 $aSILVA, P.
700 1 $aLADO, B.
700 1 $aCASTRO, A.
700 1 $aGUTIÉRREZ , L.
700 1 $aKAVANOVÁ, M.
700 1 $aQUINCKE, M.
700 1 $aBHAVANI , S.
700 1 $aRANDHAWA, M.S.
700 1 $aGERMAN, S.
773 $tMolecular Breeding, 1 August 2020, Volume 40, Issue 8, Article number 82. DOI: https://doi.org/10.1007/s11032-020-01153-5
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
08/06/2021 |
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Actualizado : |
02/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
GASTAL, G.D.A.; SCARLET D.; MELCHERT M.; ERTL R.; AURICH, C. |
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Afiliación : |
GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./ Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; DRAGOS SCARLET, Division of Obstetrics, Gynecology and Andrology, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria;; MARIA MELCHERT, Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; REINHARD ERT, Vetcore Facility, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; CHRISTINE AURICH, Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria. |
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Título : |
Epigenetic changes in equine embryos after short-term storage at different temperaturas. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Animals, 5 May 2021, Volume 11, Issue 5, Article number 1325. Open Access. Doi: https://doi.org/10.3390/ani11051325 |
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DOI : |
10.3390/ani11051325 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 16 March 2021/Accepted: 3 May 2021/Published: 6 May 2021. This work was supported by University of Veterinary Medicine Vienna, Austria. |
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Contenido : |
Simple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern.
The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and
development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer.
Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and the establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim
of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, on: (i) morphological development; (ii) expression of candidate genes associated with embryo growth and development,
maternal recognition of pregnancy, methylation and apoptosis, and (iii) gene-specific and global DNA methylation. Embryos (n = 80) were collected on day seven or day eight after ovulation and assigned to four groups: day seven control (E7F, fresh); day seven, stored for 24 h at 5 ?C (E5C);
day seven, stored for 24 h at 20 ?C (E20C) and day eight control (E8F, fresh 24h time control). The embryos and the storage medium (EquiHold, holding medium, Minitube, Tiefenbach, Germany) from all treatment groups were analyzed for (i) medium temperature, pH, and lipid peroxidation
(malondialdehyde; MDA) and (ii) embryo morphology, mRNA expression and DNA methylation (immunohistochemistry and gene-specific DNA methylation). The size of embryos stored at 5 ?C was larger (p < 0.01), whereas embryos stored at 20 ?C were smaller (p < 0.05) after 24 h. There
were no changes in pH and MDA accumulation irrespective of the group. The mRNA expression of specific genes related to growth and development (POU5F1, SOX2, NANOG), maternal recognition of pregnancy (CYP19A1, PTGES2), DNA methylation (DNMT1, DNMT3A, DNMT3B) and apoptosis
(BAX) in the E5C and E20C were either up or downregulated (p < 0.05) when compared to controls (E7F and E8F). The immune expression of 5mC and 5hmC was similar among treatment groups. Percentage of methylation in the CpG islands was lower in the specific genes ESR1, NANOG and
DNMT1 (p < 0.001) in E20C embryos when compared to E8F (advanced embryo stage). Therefore,our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Although our results suggest
some beneficial effects of storage at 20 ?C in comparison to 5 ?C, the short-term storage, regardless of temperature, modified gene expression and methylation of genes involved in embryo development and may compromise embryo viability and development after transfer. MenosSimple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern.
The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and
development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer.
Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and... Presentar Todo |
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Palabras claves : |
Development; Embryo; Embryo-maternal recognition; Equine; Methylation; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; Transport. |
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Thesagro : |
EQUINOS. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16671/1/animals-11-01325.pdf
https://www.mdpi.com/2076-2615/11/5/1325/pdf
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Marc : |
LEADER 04973naa a2200301 a 4500
001 1062116
005 2022-09-02
008 2021 bl uuuu u00u1 u #d
024 7 $a10.3390/ani11051325$2DOI
100 1 $aGASTAL, G.D.A.
245 $aEpigenetic changes in equine embryos after short-term storage at different temperaturas.$h[electronic resource]
260 $c2021
500 $aArticle history: Received: 16 March 2021/Accepted: 3 May 2021/Published: 6 May 2021. This work was supported by University of Veterinary Medicine Vienna, Austria.
520 $aSimple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer. Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and the establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, on: (i) morphological development; (ii) expression of candidate genes associated with embryo growth and development, maternal recognition of pregnancy, methylation and apoptosis, and (iii) gene-specific and global DNA methylation. Embryos (n = 80) were collected on day seven or day eight after ovulation and assigned to four groups: day seven control (E7F, fresh); day seven, stored for 24 h at 5 ?C (E5C); day seven, stored for 24 h at 20 ?C (E20C) and day eight control (E8F, fresh 24h time control). The embryos and the storage medium (EquiHold, holding medium, Minitube, Tiefenbach, Germany) from all treatment groups were analyzed for (i) medium temperature, pH, and lipid peroxidation (malondialdehyde; MDA) and (ii) embryo morphology, mRNA expression and DNA methylation (immunohistochemistry and gene-specific DNA methylation). The size of embryos stored at 5 ?C was larger (p < 0.01), whereas embryos stored at 20 ?C were smaller (p < 0.05) after 24 h. There were no changes in pH and MDA accumulation irrespective of the group. The mRNA expression of specific genes related to growth and development (POU5F1, SOX2, NANOG), maternal recognition of pregnancy (CYP19A1, PTGES2), DNA methylation (DNMT1, DNMT3A, DNMT3B) and apoptosis (BAX) in the E5C and E20C were either up or downregulated (p < 0.05) when compared to controls (E7F and E8F). The immune expression of 5mC and 5hmC was similar among treatment groups. Percentage of methylation in the CpG islands was lower in the specific genes ESR1, NANOG and DNMT1 (p < 0.001) in E20C embryos when compared to E8F (advanced embryo stage). Therefore,our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Although our results suggest some beneficial effects of storage at 20 ?C in comparison to 5 ?C, the short-term storage, regardless of temperature, modified gene expression and methylation of genes involved in embryo development and may compromise embryo viability and development after transfer.
650 $aEQUINOS
653 $aDevelopment
653 $aEmbryo
653 $aEmbryo-maternal recognition
653 $aEquine
653 $aMethylation
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aTransport
700 1 $aSCARLET D.
700 1 $aMELCHERT M.
700 1 $aERTL R.
700 1 $aAURICH, C.
773 $tAnimals, 5 May 2021, Volume 11, Issue 5, Article number 1325. Open Access. Doi: https://doi.org/10.3390/ani11051325
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